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A total number of 2635 raw movie stacks made of 50 frames each were collected with SerialEM 87 from a single individual session with a GLACIOS 200 kV FEG Cryo-TEM (ThermoFisher Scientific) using a K2 Summit detector (Gatan Inc., USA) at 36000× magnification and pixel size of 1.145 Å/pixel without pre-exposure and using a defocus of –2.6 to –1.8 µm and 50 e - /Å 2 total dose per stack (1 e - /Å 2 per frame)

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